The proposed PSO algorithm helps in correctly and quickly identifying an optimal primer pair required for a specific PCR experiment. The user-friendly interface allows (1) accession number input, (2) sequence input, and (3) input of primer constraints.

The software interface allows users to easily design fitting primer sets according to their needs. It incorporates the RefSeq database, which enables users to enter sequence accession numbers directly, or to copy/paste entire sequences in order to design primer sets. The system we introduce in this chapter is based on a particle swarm optimization (PSO) algorithm.

Examples are Primer Design Assistant (PDA) and GeneFisher. were developed, but most of these do not allow the use of sequence accession numbers for primer design. Recently, many kinds of primer design software. It is important to choose a feasible primer pair to work quickly and efficiently. The PCR technique allows a small amount of DNA to be amplified exponentially, thus ensuring that the amount of DNA is sufficient for DNA sequence analysis or gene therapy.

In recent years, polymerase chain reactions (PCR) have been widely applied in medical science.

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